Kylie Pentelow was educated at the Sir Christopher Hatton School in Wellingborough, after graduating from the University of Sheffield with an MA in journalism, her first post was as a reporter for the Bath Chronicle. Kylie Pentelow then joined BBC Points West as a video journalist, and after joining the BBC graduate training scheme undertook postings in BBC local radio in Oxford and Birmingham, before moving back to television with the BBC News Channel. Kylie Pentelow then became a reporter/presenter for BBC East Midlands Today.
Kylie Pentelow then moved to ITV becoming the North America news correspondent for the ITV Breakfast programme Daybreak, where she was primarily based in New York. In July 2013, Kylie Pentelow became a main presenter for ITV News West Country, where she currently co-anchors the 18:00 regional news alongside Ian Axton. On 3 May 2014 she made her debut presenting the Saturday evening bulletins for both ITV News London and the national ITV News programmes.